About Flexpert

On the web-version of Flexpert you can calculate the per-residue flexibility of a protein by either inputting the protein as a string or through .pdb/.fasta files.

Inputs:

Flexpert-Seq:

• Text - Enter one or more proteins according to the specified format.
• File - Select either .fasta file containing one or more proteins, or one or more .pdb files with a single-chain protein in the file.
• Note: You can only select either Text or File input options per a single prediction.

Flexpert-3D:

• File - Select one or more .pdb files with a single-chain protein in the file.

Outputs:

Files:

• Depending on your input, different output files appear:
  • A .txt file with the per-residue flexibility for all proteins always appears.
  • A .fasta file appears with all the proteins.
  • If you input a .pdb file, two .pdb files per protein appear, one with 'true' per-residue flexibilities and 'scaled' per-residue flexibilities.
  • For Flexpert-3D, another .pdb file per protein also appears containing per-residue ENM values.

Visualisations:

• You will notice that there is a possibility of seeing a visualisation of the per-residue flexibility of the provided proteins. These visualisations can only appear if you predict the flexibility via a .pdb file.
• We provide both the 'real' (flexibilities predicted by Flexpert) and the 'scaled' (flexibilities normalised according to the maximum flexibility) visualisations.
• To toggle between visualisations, click the lower-most button on the side-panel (the brush) and then choose between files.